Part:BBa_K4202007:Experience
This experience page is provided so that any user may enter their experience using this part.
Please enter
how you used this part and how it worked out.
Applications of BBa_K4202007
we transformed it into our chassis-Bacillus subtilis for further protein expression. 50ml shaking flasks with 5ml culture media were introduced to reach an extra-large bacterial population while the normal 12ml shaking bacteria tubes were used as control. From the SDS-PAGE image, we could see a clear strip locating in 120kDa’s area for the 50ml shaking flasks group, revealing the excellent function of Oxygen-limitation induced promotor. However, it seemed that only the first gene (SpaB,120kDa) had expressed under the condition, suggesting that the original sequence didn’t match well to the promotor.
See the [1] ZJU-China 2022 wiki for more details.
User Reviews
UNIQd87831c9ed17bac1-partinfo-00000000-QINU UNIQd87831c9ed17bac1-partinfo-00000001-QINU